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RCT node set-up » History » Version 3

Amber Herold, 06/21/2010 02:56 PM

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h1. RCT node set-up
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h2. Testing feature finding algorithm
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The default values in the RCT node may not work for your sample, trials should be made
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to optimize the parameters. The good parameters should find hundreds, but not thousands of
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features in your untilted image for good results.
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1.  Presets Manager> send the preset used in "Centered Square" to scope at the center
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of a square.
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2.  RCT/toolbar> Click on the "acquire" button at the far right to acquire a test image
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of which the feature finding algorithm will be applied after the acquisition.
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3.  RCT> Check the result of the numbers of feature found.  A few hundred but not
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thousands would be a good settings.
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4.  RCT/Settings> Adjust Min Feature Size (most sensitive) and/or Max Feature Size or
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the filters and then repeat 2 and 3 to get better results.
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5.  Target templates are applied to the valid raster points to create the final
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acquisition and focus targets. The template can be constant, meaning that it is defined
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relative to the image coordinate. The template can also be convolved with the raster
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points which will create multiple targets, each defined relative to the coordinate of
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the valid raster points.
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*  Min/Max Feature Size: if  < 1, it means faction of the image area.  If > 1,
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number of pixels.
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*  List of Tilts to Collect are in the order of data collection
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*  RCT node has its own drift monitoring after each tilt at the transformed focus
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target position.  Therefore, drift threshold and drift preset need to be defined.
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[[Summary of this application - MSI-RCT|< Summary of this application]] | [[Presets for MSI-RCT applications|Presets for MSI-RCT applications >]]
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