Summary of this application - MSI-Tomo¶

The Leginon "MSI-Tomography" application is originally contributed by Christian Soluway and Grant Jensen at Caltech in collaboration with David Agard and Shawn Zhang at UC San Francisco. Appion team has made some modification since. The documentation here is edited from the protocol from Lu Gan and modified to reflect the new changes.

This application uses many of the same calibration as in other MSI applications. The multiscale targeting and imaging also follows the same principle. Therefore, familiarity to the latter will help. The following links are the relevant sections in MSI applications.

MSI Quick Start checklist: A short check list for users who are familiar with the application and have saved preferences.

MSI set-up in more details: The more complicated MSI Leginon application set-up that should be completed before collecting data. Ignore the automatic hole finding set-ups since this application uses user target selection and queuing.

Queuing option: standard operation mode of "MSI-Tomography".

Trouble shooting: This trouble shooting sections addresses some of the possible challenges a Leginon user may face.