Leginon "MSI-Tomography" Application¶

Summary of this application¶

The Leginon "MSI-Tomography" application is originally contributed by Christian Soluway
and Grant Jensen at Caltech in collaboration with David Agard and Shawn Zhang at UC San
Francisco. Appion team has made some modification since. The documentation here is edited from
the protocol from Lu Gan and modified to reflect the new changes.

This application uses many of the same calibration as in other MSI applications. The
multiscale targeting and imaging also follows the same principle. Therefore, familiarity to
the latter will help. The following links are the relevant sections in MSI
applications.

MSI Quick Start checklist: A short check list for
users who are familiar with the application and have saved preferences.

<link linkend="MSISetupDetails">MSI set-up in more details</link>: The more complicated
MSI Leginon application set-up that should be completed before collecting data. Ignore the
automatic hole finding set-ups since this application uses user target selection and
queuing.

<link linkend="MSISetupDetails">Queuing option</link>: standard operation mode of
"MSI-Tomography".

<link linkend="MSI_troubleshooting">Trouble shooting</link>: This trouble shooting
sections addresses some of the possible challenges a Leginon user may face.