TiltPicker Usage¶

• Start TiltPicker
  • Extract TiltPicker, on command line type:
    

    tar -zxf tiltpicker-2.0.tgz
    

  • Go to tiltpicker directory, on command line type:
    

    cd tiltpicker
    

  • Display TiltPicker help, on command line type:
    

    ./ApTiltPicker.py -h
    

  • Open TiltPicker, on command line type:
    

    ./ApTiltPicker.py -l data/rawu048b.jpg -r data/rawu049b.jpg -t 50.0
    

• Initialize alignment:
  • Maximize window
  • Set correct zoom for images
  • Center the middle bar
  • Pick 5 partcle pairs
  • Save picks to a file
  • Hit the apply button, circles will not match crosses
  • Find theta (tilt angle)
  • Optimize inplane rotations
  • Optimize all parameters
  • Mask region to only look at overlapping regions, overlap is shown on status bar
  • Hit Auto optimize, it performs find theta, optimize angles and mask in a single click
  • If you look at the Terminal it will tell you how many optimization rounds were performed
  • Use transfer picks button, this works but is based on the alignment parameters

• Auto pick particles
  • Measure particle size using ruler tool, zoom in to get best value
  • Run DoG picker with wrong contrast
  • Reject picks
  • Run DoG picker with correct contrast
  • Show terminal
  • Accept picks
  • Look at DoG map images

• Clean up particle picks
  • Use clear bad picks button
  • Use polygon tool
  • Auto optimize
  • Use cutoff button to remove picks below a threshold
  • Add more picks
  • Save and quit