TiltPicker Usage » History » Revision 2
Revision 1 (Neil Voss, 03/17/2010 11:20 AM) → Revision 2/19 (Neil Voss, 03/17/2010 11:21 AM)
h1. TiltPicker Usage * Start TiltPicker ** Extract TiltPicker, on command line type: <pre> tar -zxf tiltpicker-2.0.tgz </pre> ** Go to tiltpicker directory, on command line type: <pre> cd tiltpicker </pre> ** Display TiltPicker help, on command line type: <pre> ./ApTiltPicker.py -h </pre> ** Open TiltPicker, on command line type: <pre> ./ApTiltPicker.py -l data/rawu048b.jpg -r data/rawu049b.jpg -t 50.0 </pre> * Initialize alignment: Within TiltPicker: ** Maximize window ** Set correct zoom for images ** Center the middle bar ** Pick 5 partcle pairs ** Save picks to a file ** Hit the apply button, circles will not match crosses ** Find theta (tilt angle) ** Optimize inplane rotations ** Optimize all parameters ** Mask region to only look at overlapping regions, overlap is shown on status bar ** Hit Auto optimize, it performs find theta, optimize angles and mask in a single click ** If you look at the Terminal it will tell you how many optimization rounds were performed ** Use transfer picks button, this works but is based on the alignment parameters * Auto pick particles ** Measure particle size using ruler tool, zoom in to get best value ** Run DoG picker with wrong contrast ** Reject picks ** Run DoG picker with correct contrast ** Show terminal ** Accept picks ** Look at DoG map images * Clean up particle picks ** Use clear bad picks button ** Use polygon tool ** Auto optimize ** Use cutoff button to remove picks below a threshold ** Add more picks ** Save and quit