Appion » TiltPicker

h1. TiltPicker Usage

* Start TiltPicker

** Extract TiltPicker, on command line type:

<pre>

tar -zxf tiltpicker-2.0.tgz

</pre>

** Go to tiltpicker directory, on command line type:

<pre>

cd tiltpicker

</pre>

** Display TiltPicker help, on command line type:

<pre>

./ApTiltPicker.py -h

</pre>

** Open TiltPicker, on command line type:

<pre>

./ApTiltPicker.py -l data/rawu048b.jpg -r data/rawu049b.jpg -t 50.0

</pre>

* Initialize alignment:

** Maximize window

** Set correct zoom for images

** Center the middle bar

** Pick 5 partcle pairs

** Save picks to a file

** Hit the apply button, circles will not match crosses

** Find theta (tilt angle)

** Optimize inplane rotations

** Optimize all parameters

** Mask region to only look at overlapping regions, overlap is shown on status bar

** Hit Auto optimize, it performs find theta, optimize angles and mask in a single click

** If you look at the Terminal it will tell you how many optimization rounds were performed

** Use transfer picks button, this works but is based on the alignment parameters

* Auto pick particles

** Measure particle size using ruler tool, zoom in to get best value

** Run DoG picker with wrong contrast

** Reject picks

** Run DoG picker with correct contrast

** Show terminal

** Accept picks

** Look at DoG map images

* Clean up particle picks

** Use clear bad picks button

** Use polygon tool

** Auto optimize

** Use cutoff button to remove picks below a threshold

** Add more picks

** Save and quit