Appion

That would be nice, but you cannot get the mean and standard deviation inside a particle box until you define a box size.

You don't have to have a box size, because we require that users define a particle size. If you define a box size that is 1.5 times the size of the particle, then it should work like I have suggested.

I see how this would be convenient, but it would slow down the particle picking process, unless you used the binned, filtered image. I guess we prefer to create sub-stacks of the original stack, but this can fill up a hard drive.

The history of this feature is that Clint created a MATLAB script that sorted particles based on their mean and standard deviation and found that particles on the periphery of the plot were over carbon had bad CTF artifacts. So, we wrote it that way.

You wouldn't have to do it during picking necessarily. You could do the stats. calculation after picking is finished. This wouldn't fix the CTF artifact problem, though. It still seems like this would be more valuable as a picking filter than it is as stack postprocessing. The earlier you can get bad picks out, the better.