Appion

here is the error msg:
Beginning Main Loop
!!! DEBUG: _startLoop imagecount=48, count=0
... Pixel size: 1.09699
!!! WARNING: overriding CTF value with user nominal value 20000.0 A

... Xmag=45579, dstep=5.00e-06, mpix=1.10e-10
!!! WARNING: Defocus minimum is less than zero
Defocus search range: 1000 A to 45000 A (0.10 to 4.50 um)
... running ctf estimation at Sat Sep 10 14:59:13 2016
/usr/local/bin/ctftilt64.exe
16sep10a_032sq_01hln_02enn-a.mrc
16sep10a_032sq_01hln_02enn-a-pow.mrc
2.7,300.0,0.07,45579.2669031,5.0,2
512,100.0,10.0,1000.0,45000.0,1000.0,500.0,-49.9953748976,10
... ctf estimation completed in 3.31 sec
Traceback (most recent call last):
File "/opt/myamisnap/bin/ctfestimate.py", line 457, in
imgLoop.run()
File "/opt/myamisnap/lib/appionlib/appionLoop2.py", line 88, in run
results = self.loopProcessImage(imgdata)
File "/opt/myamisnap/lib/appionlib/appionLoop2.py", line 146, in loopProcessImage
return self.processImage(imgdata)
File "/opt/myamisnap/bin/ctfestimate.py", line 327, in processImage
( nominaldf, self.ctfvalues['defocusinit']))
KeyError: 'defocusinit'