Appion

I was able to do one ACE run on a set of images, but when I tried it a second and third time I got the following error:

+---------+---------+--------+-------+-------+---------+ 
| Nominal | Defocus | PerErr | Conf1 | Conf2 | TotConf | 
| 2.000 | -1000000.00 | 500001.000 | -1.00 | -1.00 
| 0.000 | 
+---------+---------+--------+-------+-------+---------+ 
 ==== Committing data to database ====  
Connecting to matlab ... 
Prev best: 'acerun1', conf=0.330, defocus=-4.62 microns 
Traceback (most recent call last): 
File "/opt/appion/bin/pyace.py", line 162, in ? 
imgLoop.run() 
File "/var/tmp/appion-2.0-6_rc1-root/opt/appion/appionlib/appionLoop2.py", line 77, in run 
File "/var/tmp/appion-2.0-6_rc1-root/opt/appion/appionlib/appionLoop2.py", line 123, in loopCommitToDatabase 

File "/opt/appion/bin/pyace.py", line 112, in commitToDatabase 
apCtf.commitCtfValueToDatabase(imgdata, self.matlab, self.ctfvalue, self.params) 
File "/var/tmp/appion-2.0-6_rc1-root/opt/appion/appionlib/apCtf.py", line 44, in commitCtfValueToDatabase 

File "/usr/lib64/python2.4/shutil.py", line 47, in copyfile 
fsrc = open(src, 'rb') 
IOError: [Errno 2] No such file or directory: '/ami/exdata/appion/10may26f/ctf/acerun3/temp/im1.png'