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Ab Initio Reconstruction » History » Revision 4

Revision 3 (Anke Mulder, 05/18/2010 05:15 PM) → Revision 4/11 (Anke Mulder, 05/18/2010 05:16 PM)

h1. Ab Initio Reconstruction 

 This section contains procedures for calculating initial models from tilted and untilted datasets. 

 h2. Initial Model Procedures: 

 # [[RCT Volume|Random Conical Tilt Reconstruction]]: Obtain 3D structure from tilted data    (typically -55,0). 
 # [[OTR Volume|Orthogonal Tilt Reconstruction]]: Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45) 
 # [[EMAN Common Lines|EMAN Common Lines Reconstruction]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules. 
 # [[IMAGIC Angular Reconstitution|IMAGIC Angular Reconstitution]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules. 


 h2. Appion SideBar Snapshot: 

 !http://emg.nysbc.org/attachments/198/Picture_87.png! 

 h2. Appion Composite Output Snapshot: 

 !http://emg.nysbc.org/attachments/199/Picture_88.png! 


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 [[Particle Alignment|< Particle Alignment]] | [[Refine Reconstruction|Refine Reconstruction [[Run Alignment|Run Alignment >]] 

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