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Align Tilt-Series » History » Revision 7

Revision 6 (Alex Noble, 12/18/2015 02:09 PM) → Revision 7/8 (Alex Noble, 02/23/2017 01:40 PM)

h1. Align Tilt-Series (Appion-Protomo) 

 Appion-Protomo is a semi-automated implementation of Protomo 2.4.1, with the option of fully automated batch tilt-series processing. 

 For a technical overview of Protomo and for diagnosing Protomo-specific issues, refer to the Protomo User's Guide: http://www.electrontomography.org/?page_id=317 

 Here is a list of all YouTube links to Appion-Protomo workshops: 

 * Dec 7, 2016: https://www.youtube.com/watch?v=rDBz-l4lgyQ 

 This wiki page will soon be populated with a series of video tutorials covering: 

 # Intro video of what will be covered and where you can download everything 
 # The "This is how simple it can be!" video 
 # How Protomo aligns, Protomo output, how to tell if a tilt-series is well aligned 
 # Coarse Alignment and Summary webpages 
 # Refinement and Summary webpages 
 # Reconstruction 
 # Batch processing, fully automated mode, CTF correction, dose compensation 
 # Screening and Tilt-Series Collection tips 
 # How to use Appion-Protomo to align tilt-series that are not in the database 
 # Examples 
 # Troubleshooting