Align Tilt-Series (Appion-Protomo)¶

Appion-Protomo is a semi-automated implementation of Protomo 2.4.1, with the option of fully automated batch tilt-series processing.

For a technical overview of Protomo and for diagnosing Protomo-specific issues, refer to the Protomo User's Guide: http://www.electrontomography.org/?page_id=317

Here is a list of all YouTube links to Appion-Protomo workshops:

• Dec 7, 2016: https://www.youtube.com/watch?v=rDBz-l4lgyQ

This wiki page will soon be populated with a series of video tutorials covering:

1. Intro video of what will be covered and where you can download everything
2. The "This is how simple it can be!" video
3. How Protomo aligns, Protomo output, how to tell if a tilt-series is well aligned
4. Coarse Alignment and Summary webpages
5. Refinement and Summary webpages
6. Reconstruction
7. Batch processing, fully automated mode, CTF correction, dose compensation
8. Screening and Tilt-Series Collection tips
9. How to use Appion-Protomo to align tilt-series that are not in the database
10. Examples
11. Troubleshooting