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Auto Align Tilt Pairs » History » Revision 5

Revision 4 (Arne Moeller, 06/04/2010 09:12 AM) → Revision 5/10 (Anke Mulder, 06/04/2010 11:08 AM)

h1. Auto Align Tilt Pairs 

 Use this tool if you want to correlate and box the particles of a Random Conical Tilt Session automatically. Before you can run this program you need to pick the particles with either one of the available picking tools [[Dog Picking]], [[Manual Picking]] or [[Template Picking]]. 

 !http://emg.nysbc.org/attachments/285/Picture_86.png! Picking]]  

 h2. General Workflow: 

 # 1. Decide if you want to box the particles on all tilt angles or just one (f.e. untilted images). 
 # 

 2. Choose the picking run previously performed 
 # 

 3. Usually the default filter parameter work extremely well but feel free to play around with them. 
 # 

 4. Submit the job 
 # Once the job is finished, check the quality of tilt pair alignment using the [[Multi Img Assessment]]|Multi Image Assessment Tool]]. 

 !Screen_shot_2010-06-04_at_8.46.38_AM.png! 

 h2. Notes, Comments, and Suggestions: 



 


 [[Particle Selection|< Particle Selection]] 






 







 !Screen_shot_2010-06-04_at_8.46.38_AM.png!