Auto Align Tilt Pairs » History » Version 7
Anke Mulder, 06/04/2010 11:09 AM
| 1 | 1 | Arne Moeller | h1. Auto Align Tilt Pairs |
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| 2 | 2 | Arne Moeller | |
| 3 | 5 | Anke Mulder | Use this tool if you want to correlate and box the particles of a Random Conical Tilt Session automatically. Before you can run this program you need to pick the particles with either one of the available picking tools [[Dog Picking]], [[Manual Picking]] or [[Template Picking]]. |
| 4 | 2 | Arne Moeller | |
| 5 | 5 | Anke Mulder | !http://emg.nysbc.org/attachments/285/Picture_86.png! |
| 6 | 1 | Arne Moeller | |
| 7 | 5 | Anke Mulder | h2. General Workflow: |
| 8 | 1 | Arne Moeller | |
| 9 | 5 | Anke Mulder | # Decide if you want to box the particles on all tilt angles or just one (f.e. untilted images). |
| 10 | 7 | Anke Mulder | # Choose the picking run previously performed. |
| 11 | 5 | Anke Mulder | # Usually the default filter parameter work extremely well but feel free to play around with them. |
| 12 | 7 | Anke Mulder | # Submit the job. |
| 13 | 6 | Anke Mulder | # Once the job is finished, check the quality of tilt pair alignment using the [[Multi Img Assessment|Multi Image Assessment Tool]]. |
| 14 | 2 | Arne Moeller | |
| 15 | 5 | Anke Mulder | !Screen_shot_2010-06-04_at_8.46.38_AM.png! |
| 16 | 2 | Arne Moeller | |
| 17 | 5 | Anke Mulder | h2. Notes, Comments, and Suggestions: |
| 18 | 2 | Arne Moeller | |
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| 21 | 5 | Anke Mulder | [[Particle Selection|< Particle Selection]] |