Appion

h1. EMAN Refinement

!http://emg.nysbc.org/attachments/259/Picture_48.png!

h2. General Workflow:

# Select the CTF corrected stack of particles to use for refinement from the dropdown menu.

# Use the radio button to select the initial model to use for refinement. To upload an initial model click on the "Upload a new initial model" link or use one of the [[Import_tools|Import Tools]] in the Appion Sidebar.

# Click "Use this stack and model" to proceed to the next step.

!http://emg.nysbc.org/attachments/312/EMAN_refinement_-_select_model.png!

# Select the cluster to which you would like to submit this job.

# Check the Run Name and Output Directory.

# Set cluster parameters as is appropriate.

# double check the number of nodes and processors per node to be allocated for the reconstruction within the cluster and check wall and CPU time parameters. The "Recon procs per node" option refers to the number of *EMAN reconstruction processors* per node, NOT the amount of processors allocated to the job, which can be more.

!http://emg.nysbc.org/attachments/314/EMAN_refinement_cluster_setup.png!

# Set specific EMAN parameters. Use the mouse to point to an individual parameter, and a help-box will float above that option giving the required information for the user to choose the parameters. It is possible to set default parameters for the first iteration by clicking on the "Set Defaults for Iteration 1" box, then copy the iteration by clicking on the "copy" radio button and modifying the parameters for the subsequent iteration as necessary. Within NRAMM we have experimented with several default refinement schemes, and have provided standard parameters for GroEL-type molecules, icosahedral viruses, and mostly asymmetric molecules such as the ribosome. Specifying one of these options under the "Import Parameters" dropdown toolbox will set basic default parameters for similar molecules.

!http://emg.nysbc.org/attachments/315/EMAN-specific_parameters.png!

h2. Notes, Comments, and Suggestions:

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[[Refine Reconstruction|< Refine Reconstruction]]|[[Quality_Assessment|Quality Assessment >]]

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