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Typical Appion workflow package requirement of Appion scripts

Appion python scripts are often wrappers that calls scripts or command from other packages. List here are what is needed by each typically used options. Note that users may not want to use all functions as programs go out of fashion.

Direct Detector Frame Alignment

appion script name external package script/command within the package
makeDDAlignMotionCor2_UCSF.py MotionCor2
makeDDAlignMotionCorr_Purdue.py motioncorr v2.0 from Purdue
makeDEAlignedSum.py deProcessFrames.py
makeDEAlignedSum.py EMAN2

Automated CTF Estimation

appion script name external package
ctffind4.py CTFFIND4
gctf.py Gctf

Particle Selection

templateCorrelator.py template-based particle selection FindEM
gAutomatchFast.py template-based particle selection Gautomatch

Particle Stack Making and manipulation

appion script name subfunction package script/command within the package
general image manipulation EMAN1 proc2d
makestack2.py making Imagic stack appionlib
makestack2.py making Relion stack Relion relion_preprocess or relion_preprocess_mpi
makestack2.py CTF correction by Wiener filtering of the full image (Imagic stack only) ace2 ace2correct.exe
makestack2.py normalization by edge appionlib
makestack2.py/quickstack.py making montage of stack mean/standard deviation map ImageMagick montage
centerParticleStack.py particle centering in a stack EMAN1 centeralignint
boxMaskStack.py soft masking of helical boxed particles in the stack Spider
sortJunkStack.py sort particle by similarity to average Xmipp2 xmipp_sort_by_statistics

Updated by Anchi Cheng almost 7 years ago · 10 revisions