Frealign Refinement¶

General Workflow:¶

1. Select the non-CTF corrected stack of particles to use for refinement from the dropdown menu.
2. Select the non-CTF corrected stack of particles to use for the final reconstruction from the dropdown menu.
3. Use the radio button to select the initial model to use for refinement. To upload an initial model click on the "Upload a new initial model" link or use one of the Import Tools in the Appion Sidebar.
4. Click "Use this stack and model" to proceed to the next step.
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   h2. Notes, Comments, and Suggestions:

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