Helical Processing¶

Helical or tubular crystals, which often occur upon high-density reconstitution of membrane proteins into lipid membranes, offer some unique advantages over 2D crystals. A single image of a helical tube provides all of the information required for calculating a 3D map, and the inclusion of many tubes can be combined to improve the resolution without the need to tilt the sample in the microscope. Helical processing can be performed in real space or in Fourier space.

Helical Processing Options Available in Appion:¶

Appion used to support PHOELIX but it is no longer available. The only relevant function here is

1. Manaul Helical Picking under Feature #1771. Please refer to that for instruction in using it.

Alternatives¶

http://www.sachse.embl.de/emspring/

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