Helical Processing¶

Helical or tubular crystals, which often occur upon high-density reconstitution of membrane proteins into lipid membranes, offer some unique advantages over 2D crystals. A single image of a helical tube provides all of the information required for calculating a 3D map, and the inclusion of many tubes can be combined to improve the resolution without the need to tilt the sample in the microscope. Helical processing can be performed in real space or in Fourier space. Appion encourages the use of independent methods as every dataset is different and therefore responds differently to various protocols. In addition, the use of multiple packages can be a tool to improve reliability of reconstructions, as each method should converge on a similar result.

Helical Processing Options Available in Appion:¶

1. Helical Image Processing (PHOELIX) (Not develop actively. Use at your own risk)
2. Refinement Post-processing Procedures

Appion Sidebar Snapshot:¶

Notes, Comments, and Suggestions:¶

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