Install Xmipp¶

Install Xmipp from source¶

• Download the v2.4 source code from http://xmipp.cnb.csic.es/twiki/bin/view/Xmipp/InstallingTheSoftware
• Unzip the source code:
  

  tar zxvf Xmipp-2.4-src.tar.gz

• Go into Xmipp source directory
• Find openmpi directory
  

  locate libmpi.so
    /usr/lib64/openmpi/1.3.2-gcc/lib/libmpi.so
  

• Setup Xmipp to use openmpi by changing three lines in SConstruct
  

  cp -v SConstruct SConstruct.orig
  

  
  

  opts.Add('MPI_INCLUDE', 'MPI headers dir ', '/usr/lib/openmpi/1.2.7-gcc/include/')
  opts.Add('MPI_LIBDIR', 'MPI libraries dir ', '/usr/lib/openmpi/1.2.7-gcc/lib/')
  opts.Add('MPI_LIB', 'MPI library', 'mpi')
  

• Compile
  

  sudo mpi-selector --yes --system --set `rpm --qf '%{NAME}-%{VERSION}-gcc-%{ARCH}\n' -q openmpi`
  export PATH=$PATH:/usr/lib64/openmpi/1.3.2-gcc/bin
  ./scons.configure
  

• Look for the following line in the output:
  

  * Checking for MPI ... yes
  

• Now compile the source code
  

  ./scons.compile
  

• Move the main source code directory to global location, like /usr/local
  

  sudo mv -v Xmipp-2.4-src /usr/local/Xmipp

• Set environmental variables:
  • For bash, edit xmipp.sh:
    

    export XMIPPDIR=/usr/local/Xmipp
    export PATH=${XMIPPDIR}/bin:${PATH}
    export LD_LIBRARY_PATH=${LD_LIBRARY_PATH}:${XMIPPDIR}/lib
    

  • For C shell, edit xmipp.csh:
    

    setenv XMIPPDIR /usr/local/Xmipp
    setenv PATH ${XMIPPDIR}/bin:${PATH}
    setenv LD_LIBRARY_PATH ${LD_LIBRARY_PATH}:${XMIPPDIR}/lib
    

  • Copy to /etc/profile.d
    

    sudo cp -v xmipp.sh /etc/profile.d/
    sudo chmod 755 /etc/profile.d/xmipp.sh
    
    sudo cp -v xmipp.csh /etc/profile.d/
    sudo chmod 755 /etc/profile.d/xmipp.csh
    

Test Xmipp¶

Test Xmipp by running ml_align2d program

xmipp_ml_align2d -h

This result should appear

2104:Argument -i not found or invalid argument
File: libraries/data/args.cpp line: 502
Usage:  ml_align2d [options] 
   -i <selfile>                : Selfile with input images 
   -nref <int>                 : Number of references to generate automatically (recommended)
   OR -ref <selfile/image>         OR selfile with initial references/single reference image 
 [ -o <rootname> ]             : Output rootname (default = "ml2d")
 [ -mirror ]                   : Also check mirror image of each reference 
 [ -fast ]                     : Use pre-centered images to pre-calculate significant orientations
 [ -thr <N=1> ]                : Use N parallel threads 
 [ -more_options ]             : Show all possible input parameters 

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