Appion

h1. Install Xmipp

h2. Install Xmipp from source

We recommend installing Xmipp from source to properly use the openmpi libraries that allows you to run on multiple processors

h3. Download source code

* Download the v2.4 source code from http://xmipp.cnb.csic.es/twiki/bin/view/Xmipp/InstallingTheSoftware

* Unzip the source code:

<pre>tar zxvf Xmipp-2.4-src.tar.gz</pre>

h3. Prepare Xmipp make files

* Go into Xmipp source directory

* Find openmpi directory

<pre>

locate libmpi.so

  /usr/lib64/openmpi/1.3.2-gcc/lib/libmpi.so

</pre>

* Setup Xmipp to use openmpi by changing three lines in SConstruct

<pre>

cp -v SConstruct SConstruct.orig

</pre><pre>

opts.Add('MPI_INCLUDE', 'MPI headers dir ', '/usr/lib/openmpi/1.2.7-gcc/include/')

opts.Add('MPI_LIBDIR', 'MPI libraries dir ', '/usr/lib/openmpi/1.2.7-gcc/lib/')

opts.Add('MPI_LIB', 'MPI library', 'mpi')

</pre>

h3. Compile the source code

* Configure

<pre>

sudo mpi-selector --yes --system --set `rpm --qf '%{NAME}-%{VERSION}-gcc-%{ARCH}\n' -q openmpi`

export PATH=$PATH:/usr/lib64/openmpi/1.3.2-gcc/bin

./scons.configure

</pre>

* Look for the following line in the output:

<pre>

* Checking for MPI ... yes

</pre>

* Now compile the source code

<pre>

./scons.compile

</pre>

* Move the main source code directory to global location, like @/usr/local@

<pre>sudo mv -v Xmipp-2.4-src /usr/local/Xmipp</pre>

h3. Setup environmental variables

* For bash, edit xmipp.sh:

<pre>

export XMIPPDIR=/usr/local/Xmipp

export PATH=${XMIPPDIR}/bin:${PATH}

export LD_LIBRARY_PATH=${LD_LIBRARY_PATH}:${XMIPPDIR}/lib

</pre>

* For C shell, edit xmipp.csh:

<pre>

setenv XMIPPDIR /usr/local/Xmipp

setenv PATH ${XMIPPDIR}/bin:${PATH}

setenv LD_LIBRARY_PATH ${LD_LIBRARY_PATH}:${XMIPPDIR}/lib

</pre>

* Copy to /etc/profile.d

<pre>

sudo cp -v xmipp.sh /etc/profile.d/

sudo chmod 755 /etc/profile.d/xmipp.sh

- or -

sudo cp -v xmipp.csh /etc/profile.d/

sudo chmod 755 /etc/profile.d/xmipp.csh

</pre>

bq. You may need to log out and log back in for these changes to take place.

h2. Test Xmipp

Test Xmipp by running ml_align2d program

<pre>

xmipp_ml_align2d -h

</pre>

This result should appear

<pre>

2104:Argument -i not found or invalid argument

File: libraries/data/args.cpp line: 502

Usage:  ml_align2d [options] 

   -i <selfile>                : Selfile with input images 

   -nref <int>                 : Number of references to generate automatically (recommended)

   OR -ref <selfile/image>         OR selfile with initial references/single reference image 

 [ -o <rootname> ]             : Output rootname (default = "ml2d")

 [ -mirror ]                   : Also check mirror image of each reference 

 [ -fast ]                     : Use pre-centered images to pre-calculate significant orientations

 [ -thr <N=1> ]                : Use N parallel threads 

 [ -more_options ]             : Show all possible input parameters 

</pre>

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[[Install SPIDER|< Install SPIDER]] | [[Compile FindEM|Compile FindEM >]]

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