Particle Selection¶

The first step in single particle analysis is to pick the particles within the micrographs. Basically three main ways exist to do this, all of them are integrated within Appion. Based on the shape of the particle, the prior knowledge and the amount of data collected, the user has to make a decision which approach is the best or use different approaches simultaneously and see which works best.

1. Manual Picking¶

The manual particle picker allows the user to select the targets by eye. This can be extremely time consuming. However, if no starting model is available or the desired particles are represented in a very low concentration it is sometimes worth to spent some time selecting the particles manually. After several hundred particles have been collected a preliminary initial model or 2D averages can be generated and used as templates.

2. Template Picking¶

Template picking is usually the most accurate and convenient way to extract particles. Once an initial model or 2D averages have been acquired they can be used as templates to identify similar particles within the micrograph.

3. Dog Picking¶

Use Dog Picker if you have no accurate idea of what your particle looks like or you simply want to pick everything (this will include blobs of noise).

Notes, Comments, and Suggestions:¶

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