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Random Conical Tilt Picking » History » Version 6

Anke Mulder, 06/04/2010 10:59 AM

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h1. Random Conical Tilt Picking
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If you ran a random conical tilt session you have to pick and correlate (align particle pairs) the particles on the tilt pairs.  To find the particles you can use [[Template Picking]], [[Manual Picking]] or [[Dog Picking]]. 
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h2. To align particle pairs:
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# [[Align and Edit Tilt Pairs]]: manual alignment and editing of the tilt pairs.
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# [[Auto Align Tilt Pairs]]: automated alignment and editing of the tilt pairs.
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h2. Notes, Comments, and Suggestions:
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# We use the auto align option all the time, and it works well.  Generally, after running auto align, we check the aligned picks using the [[Multi Img Assessment]] tool in Appion.
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!http://emg.nysbc.org/attachments/282/Picture_83.png!
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!http://emg.nysbc.org/attachments/277/Picture_81.png!
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[[Particle Selection|< Particle Selection]] | [[CTF Estimation|CTF Estimation >]]
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