Project

General

Profile

Random Conical Tilt Picking » History » Revision 6

Revision 5 (Anke Mulder, 06/04/2010 10:57 AM) → Revision 6/7 (Anke Mulder, 06/04/2010 10:59 AM)

h1. Random Conical Tilt Picking 

 If you ran a random conical tilt session you have to pick and correlate (align particle pairs) the particles on the tilt pairs.    To find the particles you can use [[Template Picking]], [[Manual Picking]] or [[Dog Picking]].  

 h2. To align particle pairs: 

 # [[Align and Edit Tilt Pairs]]: manual alignment and editing of the tilt pairs. 
 # [[Auto Align Tilt Pairs]]: automated alignment and editing of the tilt pairs. 


 h2. Notes, Comments, and Suggestions: 

 # We use the auto align option all the time, and it works well.    Generally, after running auto align, we check the aligned picks using the [[Multi Img Assessment]] tool in Appion. 

 !http://emg.nysbc.org/attachments/282/Picture_83.png! 
 !http://emg.nysbc.org/attachments/277/Picture_81.png! 


 ______ 

 [[Particle Selection|< Particle Selection]] | [[CTF Estimation|CTF Estimation >]] 

 ______