Appion

h1. Random Conical Tilt Picking

If you ran a random conical tilt session you have to pick and correlate (align particle pairs) the particles on the tilt pairs.  To find the particles you can use [[Template Picking]], [[Manual Picking]] or [[Dog Picking]]. 

h2. To align particle pairs:

# [[Align and Edit Tilt Pairs]]: manual alignment and editing of the tilt pairs.

# [[Auto Align Tilt Pairs]]: automated alignment and editing of the tilt pairs.

h2. Notes, Comments, and Suggestions:

# We use the auto align option all the time, and it works well.  Generally, after running auto align, we check the aligned picks using the [[Multi Img Assessment]] tool in Appion.

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[[Particle Selection|< Particle Selection]] | [[CTF Estimation|CTF Estimation >]]

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