Stacks » History » Revision 5
Revision 4 (Anke Mulder, 05/18/2010 07:12 PM) → Revision 5/10 (Anke Mulder, 05/18/2010 07:15 PM)
h1. Stacks After particle selection, individual particles are boxed out Image formation in EM is distorted by modulation of a contrast transfer function (CTF). Distortion depends on the micrographs physical parameters of the microscope, such as keV and placed into stack files lens abberations. Correcting for further processing. these aberrations is done by comparing the experimentally observed power spectral density (PSD) of EM images to a theoretically generated CTF. h2. Available Stack Tools: # [[Stack Creation]]: Box particles selected from micrographs. Apply CTF and Astigmatism correction. [[Ace Estimation]] # [[More Stack Tools]]: Center, filter, clean-up, mask, etc particle stacks. [[Ace 2 Estimation]] # [[CtfTilt Estimation]] h2. Appion SideBar Snapshot: !http://emg.nysbc.org/attachments/211/Picture_116.png! ______ [[CTF Estimation|< CTF Estimation]] | [[Particle Alignment|Particle Alignment >]] ______