Appion

Defocal pair (Webform parameter entry only)¶

The user will need to make sure the files are organized alphabetically sorted by the group first
For example, image1.mrc=location1/defocus1, image2.mrc=location1/defocus2, image3=location2/defocus1, image4=location2/defocus2 etc.

RCT tilt pair or defocal pair (Both methods available)¶

The user will need to make sure the files are organized alphabetically sorted by the group first if web form parameter Manual entering is used.

For example, image1.mrc=location1/tilt1, image2.mrc=location1/tilt2, image3=location2/tilt1, image4=location2/tilt2 etc.

Otherwise, tilted image pairs should be need to create a parameter file to incorporate the stage alpha tilt information during upload.

Tomography tilt series (Parameter File method only)¶

The file entry should be ordered by the tilt series. It is possible to upload a bi-directional (0 to positive tilts and then 0 to negative tilts, for example) tilt series.
Number of images in the tilt group defines the tilt series.