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FEI TecnaiTitan installation specifics » History » Version 25

Anchi Cheng, 01/29/2018 04:25 PM

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h1. FEI Tecnai Titan installation specifics
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h2. Install win32 version of python subpackages
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Use the 32-bit Windows files (those marked as win32 and certainly not the ones says 64) in http://emg.nysbc.org/projects/leginon/files for these.
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Here is the full list
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{{include(Windows_32_External_Package_Installers_Common)}}
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{{include(Comtypes_installer)}}
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h2. Required scripting from FEI
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|TEM Scripting or Tecnai Scripting|Request this when purchasing the microscope|Required|
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|TomMoniker Scripting|Should come with TEM Scripting|Required|
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|Low Dose Server Library|Should come with the microscope if low dose kit was purchased|Required only if you want to use low dose kit with Leginon's Manual Application|
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h3. Run checkcom.py
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* From a command line window:
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<pre>cd C:\python27\Lib\site-packages\pyscope
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C:\python27\python.exe checkcom.py</pre>
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* The output should at least find typelib for
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<pre>
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TEM Scripting
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</pre>
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and
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<pre>
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TOM Moniker
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</pre>
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If you have Low Dose Kit
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<pre>
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Tecnai Low Dose Kit
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</pre>
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You will only find Tecnai Exposure Adaptor (Scripting for film exposure) if you ask FEI for it.
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h2. instruments.cfg
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cs is *constant for sperical abberation*
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For Tecnai series:
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<pre>
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[TEM]
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class: fei.Tecnai
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cs: 2.0e-3
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</pre>
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For Halo (Titan column with tecnai compustage):
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<pre>
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[TEM]
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class: fei.Halo
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cs: 2.7e-3
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</pre>
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For Krios (different compustage)
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<pre>
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[TEM]
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class: fei.Krios
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cs: 2.7e-3
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</pre>
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For Krios EF mode, treated as a separate instrument. i.e. add
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<pre>
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[TEM]
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class: fei.EFKrios
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cs: 2.7e-3
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</pre>
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h2. Setup fei.cfg
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{{include(setup fei.cfg)}}
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h2. Testing with pyscope
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For Tecnai series In python command
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<pre>
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from pyscope import fei
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t = fei.Tecnai()
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t.getMagnification()
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</pre>
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For Krios
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<pre>
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from pyscope import fei
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t = fei.Krios()
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t.getMagnification()
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</pre>
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For Krios with EF-TEM mode, add also
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<pre>
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from pyscope import fei
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t = fei.EFKrios()
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t.getMagnification()
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</pre>
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You should get the current magnification at the microscope when the main viewing screen is up
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h2. Trouble Shooting
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[[unable to initializ Tecnai interface error]]
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h2. Programs to open before Leginon Client: None