Acquire images on Falcon II¶

Preparation in TUI/TIA¶

Acquire Gain/Dark References¶

Follow FEI's instruction in obtaining Gain/Dark References. If you plan to save frames, make sure you repeat the process with "Store intermediate images" option on.

Recommended dose rate¶

The optimal dose rate is likely between 3 - 3.5 electrons/pixel/frame or 50-60 electron/pixel/s based on available literature of high resolution structures using this camera.

Preset Recommendation¶

An example can be found in Pre-MSI_Set-up