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Falcon acquire image » History » Revision 2

Revision 1 (Anchi Cheng, 07/22/2014 08:09 PM) → Revision 2/6 (Anchi Cheng, 07/23/2014 03:36 PM)

h1. Acquire images on Falcon II 

 h2. Preparation in TUI/TIA 

 h3. Acquire Gain/Dark References 

 Follow FEI's instruction in obtaining Gain/Dark References.    If you plan to save frames, make sure you repeat the process with "Store intermediate images" option on. 

 h2. Recommended dose rate 

 The optimal dose rate is likely between 3 - 3.5 electrons/pixel/frame or 50-60 electron/pixel/s based on available literature of high resolution structures using this camera. 

 h2. Preset Recommendation 

 An example can be found in [[Pre-MSI_Set-up#Preset-Design-Example-for-Falcon-II-on-Krios]]