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Krios1-K2-NYSBC-large shift » History » Version 1

Anchi Cheng, 03/21/2019 03:31 PM

1 1 Anchi Cheng 
h1. Krios1-K2-NYSBC-large shift
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h1. Krios-K2 Presets used for 1.9 A mouse recombinant apoferritin reconstruction data collection at SEMC @ NYSBC





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The same as [[Krios1-K2-NYSBC]] except





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|Probe|_.Lens Series|Magnification:|_.Preset name:|_.Image Shift (x,y):|_.Dimension:|_.Binning:|_.Camera Dose (e/camera pixel/s):|_.Exposure Time (ms):|_.Specimen Dose Rate (e/A^2):|_.Spot Size:|_.Defocus (m):|_.C2 (um):|frame saving:|Camera|





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|nano|SA|1300**|hl|Aligned|927x959|4|8|200|0.004|11|-1.5e-4|70|no|K2|





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|nano|SA|22500|en|0,0|3710x3838|1|4.2 e/pixel/s and ~1.12 um on the specimen|6000|58|6|(-1e-6 to -2e-6)|70|200 ms/frame|





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**These two magnifications are add-on to standard SA lens series made for NYSBC Krios1. May not be available at other scopes.





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* Note: no defocal pair was acquired in this experiment.  Therefore, no ef preset.