Krios1-K2-NYSBC-large shift » History » Version 5
Anchi Cheng, 03/21/2019 06:45 PM
| 1 | 1 | Anchi Cheng | h1. Krios1-K2-NYSBC-large shift |
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| 3 | h1. Krios-K2 Presets used for 1.9 A mouse recombinant apoferritin reconstruction data collection at SEMC @ NYSBC |
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| 5 | The same as [[Krios1-K2-NYSBC]] except |
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| 7 | |Probe|_.Lens Series|Magnification:|_.Preset name:|_.Image Shift (x,y):|_.Dimension:|_.Binning:|_.Camera Dose (e/camera pixel/s):|_.Exposure Time (ms):|_.Specimen Dose Rate (e/A^2):|_.Spot Size:|_.Defocus (m):|_.C2 (um):|frame saving:|Camera| |
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| 8 | 5 | Anchi Cheng | |nano|SA|1300**|hl|Aligned|927x959|4|8|1200|0.01|11|-6e-5|70|no|K2| |
| 9 | 2 | Anchi Cheng | |nano|SA|37000|en|0,0|3710x3838|1|4.2 e/pixel/s and ~1.12 um on the specimen|6000|58|6|(-1e-6 to -2e-6)|70|200 ms/frame|K2| |
| 10 | 1 | Anchi Cheng | |
| 11 | 3 | Anchi Cheng | **This magnification is an add-on to standard SA lens series made for NYSBC Krios1. May not be available at other scopes. |
| 12 | 1 | Anchi Cheng | |
| 13 | * Note: no defocal pair was acquired in this experiment. Therefore, no ef preset. |