Electron Microscopy Group

09/03/2021

08:27 PM Leginon Leginon: RE: Error acquiring image: Error sending request: Request

Well, now there are errors at different places.
Here is a screenshot of the error log and the client log after runn... Caleigh Azumaya

08:05 PM Leginon Leginon: RE: Error acquiring image: Error sending request: Request

with save frames Caleigh Azumaya

08:01 PM Leginon Leginon: RE: Error acquiring image: Error sending request: Request

Oh yes, specifically, it will take
Raw, Channel 0, Acquire = no error
Dark, Both Channels, Acquire = error
then ... Caleigh Azumaya

07:37 PM Leginon Leginon: RE: Error acquiring image: Error sending request: Request

Hi Anchi,
Thank you so much. The pyscope test is attached below. Let me know if you need anything else to help di... Caleigh Azumaya

05:35 PM Leginon Leginon: Error acquiring image: Error sending request: Request

Hi,
We have been using leginon 3.5 for the last 6 months and things were working until I updated SerialEM on our ... Caleigh Azumaya

05/10/2021

01:04 PM Leginon Leginon: RE: K3 sensitivity value very low

Yes great! Thank you so much, Anchi. The image looked very dark before. Now the sensitivity is 0.969 just like it sho... Caleigh Azumaya

05/07/2021

11:10 PM Leginon Leginon: RE: K3 sensitivity value very low

Sorry, yes I mean the ratio between leginon counts/GMS electrons. It's very low when I use either CDS or when I use n... Caleigh Azumaya

08:40 PM Leginon Leginon: RE: K3 sensitivity value very low

OK thank you! If it helps, I am having the same sensitivity/ratio issue with CDS and without. Caleigh Azumaya

01:08 PM Leginon Leginon: RE: K3 sensitivity value very low

I am in the Navigation node and I sent my en preset to do the reading (it is 8eps in GMS at GMS bin1). The mean count... Caleigh Azumaya

05/06/2021

07:59 PM Leginon Leginon: K3 sensitivity value very low

While doing dose calibration (camera sensitivity) for our Glacios/K3 I am getting an extremely low number that I don'... Caleigh Azumaya