Appion

User on the microscope has a preset set at -2 um for nominal defocus, but that is not the value that is provided to CTFFind4. Given that they are doing a tilt data collection, does Appion try to compensate?

For example:

Filename: 17mar15a_grid1_00028gr_00001sq_v02_00002hln_v01_00002enn-a.mrc
Size: 54.32 MB
Acquired: 2017-15-03 10:07:18
Path: /gpfs/leginon/ygomez/17mar15a/rawdata/
Session: 17mar15a - polZ no det, tilt 30-40
Instrument: Krios - GatanK2Counting
Scope Host: titan52334860

en preset:

mag: 22500 defocus: -2.00 μm pixelsize: 1.10 Å dose: 74.48 e–/Å2 tilt: -30.0°

en-a preset - CTFFind4 estimation:

runname: ctffind4run2 nomDef: -2.98 μm def1: 3.06 μm def2: 3.11 μm θastig: -60.78 amp con: 7.00E-2 cs: 2.70
res (0.8): 7.28 res (0.5): 7.07 conf (appion): 0.98 res (pkg): 6.93 Å conf (pkg): 0.98

In the Image Data tree:
Data > scope > defocus = -2.98 um
Data > scope > focus = -0.00254749943596904

Data > preset > defocus = -2.00 um