Ab Initio Reconstruction » History » Version 5
Anke Mulder, 05/18/2010 05:18 PM
| 1 | 1 | Anke Mulder | h1. Ab Initio Reconstruction |
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| 3 | This section contains procedures for calculating initial models from tilted and untilted datasets. |
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| 5 | 5 | Anke Mulder | h2. Tilted Data Procedures: |
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| 7 | # [[RCT Volume|Random Conical Tilt Reconstruction]]: Obtain 3D structure from tilted data (typically -55,0). |
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| 8 | # [[OTR Volume|Orthogonal Tilt Reconstruction]]: Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45) |
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| 11 | h2. Un-Tilted Data Procedures: |
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| 13 | 1 | Anke Mulder | # [[EMAN Common Lines|EMAN Common Lines Reconstruction]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules. |
| 14 | # [[IMAGIC Angular Reconstitution|IMAGIC Angular Reconstitution]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules. |
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| 16 | h2. Appion SideBar Snapshot: |
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| 18 | 2 | Anke Mulder | !http://emg.nysbc.org/attachments/198/Picture_87.png! |
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| 20 | h2. Appion Composite Output Snapshot: |
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| 22 | 3 | Anke Mulder | !http://emg.nysbc.org/attachments/199/Picture_88.png! |
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| 25 | ______ |
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| 27 | 4 | Anke Mulder | [[Particle Alignment|< Particle Alignment]] | [[Refine Reconstruction|Refine Reconstruction >]] |
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| 29 | ______ |