Ab Initio Reconstruction » History » Revision 5
Revision 4 (Anke Mulder, 05/18/2010 05:16 PM) → Revision 5/11 (Anke Mulder, 05/18/2010 05:18 PM)
h1. Ab Initio Reconstruction This section contains procedures for calculating initial models from tilted and untilted datasets. h2. Tilted Data Initial Model Procedures: # [[RCT Volume|Random Conical Tilt Reconstruction]]: Obtain 3D structure from tilted data (typically -55,0). # [[OTR Volume|Orthogonal Tilt Reconstruction]]: Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45) h2. Un-Tilted Data Procedures: # [[EMAN Common Lines|EMAN Common Lines Reconstruction]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules. # [[IMAGIC Angular Reconstitution|IMAGIC Angular Reconstitution]]: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules. h2. Appion SideBar Snapshot: !http://emg.nysbc.org/attachments/198/Picture_87.png! h2. Appion Composite Output Snapshot: !http://emg.nysbc.org/attachments/199/Picture_88.png! ______ [[Particle Alignment|< Particle Alignment]] | [[Refine Reconstruction|Refine Reconstruction >]] ______