Ab Initio Reconstruction¶

This section contains procedures for calculating initial models from tilted and untilted datasets.

Tilted Data Procedures¶

1. Random Conical Tilt Reconstruction (RCT Volume): Obtain 3D structure from tilted data (typically -55,0).
2. Orthogonal Tilt Reconstruction (OTR Volume): Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45)

Un-Tilted Data Procedures¶

1. EMAN Common Lines Reconstruction: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.
2. IMAGIC Angular Reconstitution: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.

Appion SideBar Snapshot:¶

Appion Composite Output Snapshot:¶

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