Ab Initio Reconstruction¶

This section contains procedures for calculating initial models from tilted and untilted datasets.

Tilted Data Procedures¶

1. Random Conical Tilt Reconstruction: Obtain 3D structure from tilted data (typically -55,0).
2. Orthogonal Tilt Reconstruction: Obtain a 3D structure from data collected at 2 tilts (typically -45,0,45)

Un-Tilted Data Procedures¶

1. EMAN Common Lines Reconstruction: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.
2. IMAGIC Angular Reconstitution: Obtain a 3D structure from untilted 2D data containing randomly oriented molecules.

Appion SideBar Snapshot:¶

Appion Composite Output Snapshot:¶

< Particle Alignment | Refine Reconstruction >