Install EMAN¶

EMAN1 is a fundamental package used in appion for general file conversion and image filtering.

Download latest EMAN1 (not EMAN2) binaries (currently version 1.9):¶

1. Go to the EMAN webpage
2. Click on (download) next to EMAN1
3. Scroll to Download 1.9
4. Click on Download 1.9 link
5. Select the appropriate package
   • we recommend using the cluster version of EMAN1
   • x86 for 32bit and x86_64 for 64bit machines
   • In this case, we would download the "eman-linux-x86_64-cluster-1.9.tar.gz"
6. Go to download directory and unzip the tar file:
   

   tar -zxvf eman-linux-x86_64-cluster-1.9.tar.gz

7. Move the unzipped folder to a global location
   

   sudo mv -v EMAN /usr/local/

8. Run the EMAN installer
   

   cd /usr/local/EMAN/
   ./eman-installer
   

9. Set enivromental variables
   • For BASH, create an eman.sh and add the following lines:
     

     export EMANDIR=/usr/local/EMAN
     export PATH=${EMANDIR}/bin:${PATH}
     export LD_LIBRARY_PATH=${LD_LIBRARY_PATH}:${EMANDIR}/lib
     export PYTHONPATH=${EMANDIR}/lib
     

   • For C shell, create an eman.csh and add the following lines:
     

     setenv EMANDIR /usr/local/EMAN
     setenv PATH ${EMANDIR}/bin:${PATH}
     setenv LD_LIBRARY_PATH ${EMANDIR}/lib
     setenv PYTHONPATH ${EMANDIR}/lib
     

   • And then add it to the global /etc/profile.d/ folder
     

     sudo cp -v eman.sh /etc/profile.d/eman.sh
     sudo chmod 755 /etc/profile.d/eman.sh
     
     sudo cp -v eman.csh /etc/profile.d/eman.csh
     sudo chmod 755 /etc/profile.d/eman.csh
     

Test EMAN install¶

Run proc2d

proc2d test.mrc test.mrc apix=2.0 addnoise=1.0 clip=64,64

Should create 64x64 image of noise, to view the file:

v2 test.mrc

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