Install Grigorieff lab software¶

The Grigorieff lab at Brandies provides several individual programs that are used in Appion. See their main software page to download their software

Software used in Appion:¶

• Frealign — Refinement of 3D reconstructions using single-particle images
• CTFfind and CTFtilt — CTF estimation
  • NOTE: Once installed, you must rename ctffind to ctffind4 and ctftilt.exe to ctftilt64.exe or create symbolic links. Otherwise, you will receive an error that ctffind64.exe was not found during ctf estimation.
• Signature — Particle selection from micrographs
  • NOTE: Once installed, you must rename signature.exe to signature64.exe or create symbolic links. Otherwise, you will receive an error that signature64.exe was not found.
• Rmeasure — Resolution measurement

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