Install Grigorieff lab software¶

The Grigorieff lab at Brnadies provides several individual programs that are used in Appion. See their main software page

Software used in Appion:¶

Frealign: Refinement of 3D reconstructions using single-particle images¶

• Grigorieff, N. 2007. FREALIGN: high-resolution refinement of single particle structures. J Struct Biol. 157:117–125.
• Grigorieff, N. 1998. Three-dimensional structure of bovine NADH:ubiquinone oxidoreductase (complex I) at 22 A in ice. J Mol Biol. 277:1033-46.
• Wolf, M, DeRosier DJ, Grigorieff N. 2006. Ewald sphere correction for single-particle electron microscopy. Ultramicroscopy. 106:376-82.
• Stewart, A, Grigorieff N. 2004. Noise bias in the refinement of structures derived from single particles. Ultramicroscopy. 102:67-84.

Ctffind3 & ctftilt: CTF estimation¶

• Mindell, JA, Grigorieff N. 2003. Accurate determination of local defocus and specimen tilt in electron microscopy. J Struct Biol. 142:334-47.

Signature: Particle selection from micrographs¶

• Chen, JZ, Grigorieff N. 2007. SIGNATURE: a single-particle selection system for molecular electron microscopy. J Struct Biol. 157:168-73.

Rmeasure: Resolution measurement¶

• Grigorieff, N. 2000. Resolution measurement in structures derived from single particles. Acta Crystallogr D Biol Crystallogr. 56:1270-7.
• Sousa, D, Grigorieff N. 2007. Ab initio resolution measurement for single particle structures. J Struct Biol. 157:201-10.
  

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