Continuous tilt electron diffraction recording » History » Version 12
  Anchi Cheng, 04/29/2020 05:14 PM 
  
| 1 | 6 | Anchi Cheng | h1. Continuous tilt electron diffraction recording use Ceta or Ceta-D camera and TFS scopes | 
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| 2 | 1 | Anchi Cheng | |
| 3 | Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt. Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release. As is, it is only fully implemented for TFS Ceta or Ceta-D camera. | ||
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| 5 | Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places. | ||
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| 7 | h2. Components of the feature | ||
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| 9 | 5 | Anchi Cheng | # Diffraction mode TEM instrument | 
| 10 | 8 | Anchi Cheng | # Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut | 
| 11 | 9 | Anchi Cheng | # Rolling-shutter movie acquisition using TIA interface | 
| 12 | # Data conversion and upload of the movies into Leginon database and crystallography format | ||
| 13 | 4 | Anchi Cheng | # [[ MSI-Diffr application ]] | 
| 14 | 11 | Anchi Cheng | # [[Camera length calibration]] | 
| 15 | 1 | Anchi Cheng | |
| 16 | h2. Installation (assuming that you've already have Leginon installation). | ||
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| 18 | # Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed). | ||
| 19 | 12 | Anchi Cheng | # Install or set environment to use git branch myami-ed or current myami-beta on TFS microscope with Ceta camera as well as your leginon linux box. | 
| 20 | 10 | Anchi Cheng | # import updated Calibrations application (see [[Steps_involved_in_the_installation]]) | 
| 21 | # import MSI-diffraction and settings from your_myami/leginon/applications (see general description of [[Steps_involved_in_the_installation]] regarding importing application and additional settings for the application) | ||
| 22 | 5 | Anchi Cheng | # [[Setup Diffraction mode TEM instrument]] | 
| 23 | 7 | Anchi Cheng | # [[Setup Ceta to use Advanced TEM Scripting]] | 
| 24 | 3 | Anchi Cheng | # [[AutoIt program and script compilation]] | 
| 25 | 2 | Anchi Cheng | |
| 26 | h2. Usage | ||
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| 28 | (To be written) |