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Continuous tilt electron diffraction recording » History » Version 12

Anchi Cheng, 04/29/2020 05:14 PM

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h1. Continuous tilt electron diffraction recording use Ceta or Ceta-D camera and TFS scopes
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Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt.  Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release.  As is, it is only fully implemented for TFS Ceta or Ceta-D camera.
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Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places.
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h2. Components of the feature
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# Diffraction mode TEM instrument
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# Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut
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# Rolling-shutter movie acquisition using TIA interface
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# Data conversion and upload of the movies into Leginon database and crystallography format
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# [[ MSI-Diffr application ]]
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# [[Camera length calibration]]
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h2. Installation (assuming that you've already have Leginon installation).
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# Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed).
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# Install or set environment to use git branch myami-ed or current myami-beta on TFS microscope with Ceta camera as well as your leginon linux box.
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# import updated Calibrations application (see [[Steps_involved_in_the_installation]])
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# import MSI-diffraction  and settings from your_myami/leginon/applications (see general description of [[Steps_involved_in_the_installation]] regarding importing application and additional settings for the application)
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# [[Setup Diffraction mode TEM instrument]]
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# [[Setup Ceta to use Advanced TEM Scripting]]
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# [[AutoIt program and script compilation]]
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h2. Usage
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(To be written)