Leginon

h1. Continuous tilt electron diffraction recording use Ceta or Ceta-D camera and TFS scopes

Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt.  Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release.  As is, it is only fully implemented for TFS Ceta or Ceta-D camera.

Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places.

h2. Components of the feature

# Diffraction mode TEM instrument

# Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut

# Rolling-shutter movie acquisition using TIA interface

# Data conversion and upload of the movies into Leginon database and crystallography format

# [[ MSI-Diffr application ]]

# [[Camera length calibration]]

h2. Installation (assuming that you've already have Leginon installation).

# Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed).

# Install or set environment to use git branch myami-ed or current myami-beta on TFS microscope with Ceta camera as well as your leginon linux box.

# import updated Calibrations application (see [[Steps_involved_in_the_installation]])

# import MSI-diffraction  and settings from your_myami/leginon/applications (see general description of [[Steps_involved_in_the_installation]] regarding importing application and additional settings for the application)

# [[Setup Diffraction mode TEM instrument]]

# [[Setup Ceta to use Advanced TEM Scripting]]

# [[AutoIt program and script compilation]]

h2. Usage

h3.  Confirm that instruments.cfg is properly set up to use feicam for Ceta camera.  Especially if you use Falcon camera on the same instrument for imaging.

h3. Start MSI-DIffr application and assign client as required.

h3. [[Setup df preset]]