Leginon

*cs is the coefficient of spherical aberration in meters*.  Find out from TEM manufacture the proper value for your tem.

For example

<pre>

[tem]

class: tecnai.Tecnai

cs: 2.0e-3

</pre>

* height and width is based on the orientation presented in Leginon image viewer.

* Some Tietz camera may have an odd physical dimension.  Use TCL/EMMenu program to find out the good dimension used in Tietz's own software and entered in this configuration.

For example

<pre>

[camera]

class: gatan.Gatan

zplane: 50

height: 4096

width: 4096

</pre>

*zplane is a number that gives the order of camera*.  If a camera with low zplane value is going to acquire an image, Leginon will attempt to retract all cameras at higher zplane values.

!zplane.png!

* One computer for both tem and digital camera:

<pre>

[tem]

class: tecnai.Tecnai

cs: 2.0e-3

[camera]

class: gatan.Gatan

zplane: 50

height: 4096

width: 4096

</pre>

* Separate computers for the two instruments:  configure only the instrument reside on the particular computer

** On the computer that controls the tem:

<pre>

[tem]

class: tecnai.Tecnai

cs: 2.0e-3

</pre>

** On the computer that controls the digital camera:

<pre>

[camera]

class: gatan.Gatan

zplane: 50

height: 4096

width: 4096

</pre>

* Two cameras that retract/insert at the same time, typically one of them is an off-axis camera, such as in the case of DE12 and DE Survey camera pair.

** On the computer that controls the digital camera:

<pre>

[camera1]

class: de.DE12

zplane: 50

height: 3072

width: 4096

[camera2]

class: de.DESurvey

zplane: 50

height: 2048

width: 2048

</pre>

* Two cameras on the same computer  but one is retractable and one is non-retractable and at lower position.

** On the computer that controls the digital camera:

<pre>

[camera1]

class: de.DE12

zplane: 50

height: 3072

width: 4096

[camera2]

class: tietz.TietzPXL

zplane: 5

height: 4096

width: 4096

</pre>

* Gatan Orius camera that is installed typically with Gatan K2/Falcon camera is technically on the same plane but is not capable of insertion unless K2 is retracted.  On the other hand, K2 camera insertion includes a retraction of Orius at lower level of the function call in DM.  Therefore, Orius is considered as a camera at lower zplane.

<pre>

[camera1]

class: dmsem.GatanK2Base

zplane: 50

height: 3838

width: 3710

[camera2]

class: dmsem.GatanOrius

zplane: 49

height: 2048

width: 2048

</pre>