Leginon Manual¶

• Table of contents
• Leginon Manual
  • 1. An introduction to Leginon
  • 2. Leginon System version 1.6
  • 3. Update to Leginon System version 1.6
  • 4. Complete Installation
  • 5. Leginon Administration Tools
  • 6. Using Project Management Tools
  • 7. Installation Troubleshooting
  • 8. Microscope Set-up
  • 9. Start Leginon
  • 10. Leginon "Manual Application"
  • 11. Leginon "Calibrations" Application
  • 12. Multi-Scale Imaging Applications in General
  • 13. Leginon "MSI-Edge" Application
  • 14. Leginon "MSI-T" Application
  • 15. Leginon "MSI-raster" Application
  • 16. Leginon "MSI-RCT" and MSI-RCT raster" Applications
  • 17. Leginon "RobotMSI-Section" Application
  • 18. Leginon "MSI-Tomography" Application
  • 19. Leginon "Robot-MSI-Screen" Applications
  • 20. Frequent Asked Questions
  • 21. Trouble shooting
  • 22. Initial MSI application preference setup
  • 23. Node Descriptions
  • 24. Create and Edit Applications

1. An introduction to Leginon¶

1. What is the Leginon System?
2. Terminology
3. Graphical User Interface
4. Minimum Requirements and current NRAMM setup
5. Getting Started

2. Leginon System version 1.6¶

1. pyScope Change
2. New User Features
3. Updated Applications (All)
4. New Project Web Tool Features
5. New dbemtool Web Tool Features (1.5.1)
6. Bug Fixes
7. Recommended Application Preference Changes when updated from version 1.5
8. Known Bugs

3. Update to Leginon System version 1.6¶

1. Update from v1.1 and v1.2 NOT AVAILABLE
2. Update from v1.3-Follow v1.4 update after performing these
3. Update from v1.4
4. How to Update from v1.5 (Linux)
5. Import the new (1.6) applications included with Leginon
6. How to Update from v1.5 (Window)
7. Additional work at the computer controlling the microscope and camera (Window)
8. Recommended Application Preference Changes when updated from version 1.5

4. Complete Installation¶

1. Four Parts of the Leginon System
2. What is in this Chapter
3. Possible Computer Set-up Configurations
4. Ports used by Leginon
5. Where to register and download Leginon
6. Processing Server Installation
7. Database Server Installation
8. Web Server Installation
9. Windows Installation
10. Additional installation on the microscope computer
11. Next Steps
12. Backup Practices

5. Leginon Administration Tools¶

1. Recommandation for setup at a new institute
2. Steps involved in the installation
3. Set up for a new regular user
4. More about Groups
5. More about Instruments
6. Applications
7. Calibrations
8. Goniometer

6. Using Project Management Tools¶

1. Recommendataion-Dividing projects by long-term goal
2. Installation
3. Go to project tools page
4. Add a new project
5. Edit an existing project
6. Grid management

7. Installation Troubleshooting¶

1. Getting Help
2. Installation/Update problems
3. Administration Tool problems
4. Test run operation problems
5. Network problems
6. Troubles with Imaging
7. Troubles with Calibrations

8. Microscope Set-up¶

1. Good Alignments Save Time
2. Microscope Set-up Checklist

9. Start Leginon¶

1. Test Leginon with Simulator
2. Test Leginon on the Computer Controlling the Microscope
3. Test Network Connection Between Remote and Microscope Computers
4. Run Leginon Client on the Microscope Computer
5. Start Leginon at your main working station remotely

10. Leginon "Manual Application"¶

1. The Application
2. Startup
3. Running "Manual" Application
4. Advanced Features
5. Using the Web viewer

11. Leginon "Calibrations" Application¶

1. Importance of making calibrations
2. Grids for Calibration
3. Startup
4. Creating Presets for the first time
5. Calibration without presets
6. Pixel Size Calibration
7. Bright and Dark reference images
8. Image Shift matrix calibration
9. Beam Shift matrix calibration
10. Stage Position matrix calibration
11. Modeled Stage Position calibration
12. Checking Matrix and Modeled Stage Position Calibration
13. Autofocus Calibration with Beam Tilt Node
14. Dose (Camera Sensitivity) Calibration

12. Multi-Scale Imaging Applications in General¶

1. Multi-Scale Imaging Concept
2. Ways of Moving to Targets in Nodes that acquire images inside MSI
3. Flavors of MSI applications
4. Summary of MSI applications
5. Pre-MSI Set-up
6. Initial MSI application preferences
7. Special Operation Preference setup
8. MSI Quick-start
9. MSI set-up in more details
10. Special Operation setup
11. MSI Operation
12. Queuing option
13. Queuing Example 1 - Exposure Targeting
14. Queuing Example 2 - Hole Targeting only
15. Queuing Example 3 - Both Exposure and Hole Targeting
16. Monitor Progress of Queued Targets

13. Leginon "MSI-Edge" Application¶

1. Summary of this application
2. Hole Targeting Set-up for MSI-Edge
3. Exposure Targeting Set-up for MSI-Edge

14. Leginon "MSI-T" Application¶

1. Summary of this application
2. Creating Template
3. JAHC finder nodes set-up
4. Queuing Option

15. Leginon "MSI-raster" Application¶

1. Summary of this application
2. raster finder nodes set-up
3. Queuing Option
4. Improving Autofocusing

16. Leginon "MSI-RCT" and MSI-RCT raster" Applications¶

1. Summary of these applications
2. RCT node set-up
3. Presets for MSI-RCT applications

17. Leginon "RobotMSI-Section" Application¶

1. Summary of this application
2. Raster Generation node set-up
3. Other special preference set-up
4. Tips on Operation

18. Leginon "MSI-Tomography" Application¶

1. Summary of this application
2. Set-up Before Running
3. Running the application
4. Full Protocol on a F30 with an energy filter

19. Leginon "Robot-MSI-Screen" Applications¶

1. Summary of these applications
2. Robot node set-up
3. 1st Pass application
4. Evaluation application
5. 2nd Pass application
6. Operation

20. Frequent Asked Questions¶

1. How does Leginon Name the Files?
2. Why does the first Grid Image Not Numbered 00001?
3. What is the definition of ice thickness in the hole finders?

21. Trouble shooting¶

1. Getting Help
2. Do and Do Not in leginon
3. Pausing and Aborting during data collection
4. General operation problems
5. Hardware Troubles
6. Troubles with Imaging
7. Troubles with Targeting
8. Troubles with Focusing and Drift Check
9. Troubles with Calibrations
10. Troubles with Tomography

22. Initial MSI application preference setup¶

1. MSI-Tomography Preferences
2. Robot MSI-Section preference set-up

23. Node Descriptions¶

1. Acquisition
2. BeamFixer
3. Beam Tilt Calibrator
4. Beam Tilt Imager
5. Click Target Finder
6. Corrector
7. Dose Calibrator
8. Drift Manager
9. The EM node
10. FFT Maker
11. Focuser
12. Gon Modeler
13. Hole Finder
14. Hole Depth
15. JAHC Hole Finder (Template Hole Finder)
16. Manual Acquisition
17. Matlab Target Finder
18. Matrix Calibrator
19. Mosaic Click Target Finder
20. Mosaic Target Maker
21. Navigator
22. Pixel Size Calibrator
23. Presets Manager
24. Raster Finder
25. Raster Filter
26. RCT
27. Robot
28. RobotAtlasTargetFinder
29. Tomography
30. Transform Manager

24. Create and Edit Applications¶

1. Use the Application Editor to create Leginon applications
2. Reload and Edit an existing application
3. Edit an existing application as an xml file
4. Create Leginon "Simple Application"
5. "Calibrations" Application
6. "MSI-Edge" Application
7. "MSI-T" Application
8. "MSI-raster" Application
9. "MSI-Tomography" Application
10. "Manual" Application