Electron Microscopy Group

03/18/2016

05:24 PM Leginon Appion: The fit from CTF estimation using CTFFIND4 is much worse than when using CTFFIND3

Hi!
I ran both CTFFIND3 and CTFFIND4 on a set of negatively stained images. On the SAME negatively stained image... @Maria Janssen

12/02/2015

04:08 PM Leginon Leginon: RE: Stage does not rotate back to zero after doing z-height measurements

Thanks, an FEI engineer looked at it today and according to him the microscope stage is tilting perfectly to exactly ... @Maria Janssen

03:12 PM Leginon Leginon: RE: Stage does not rotate back to zero after doing z-height measurements

Hi Anchi,
That's what I thought... however when we ask FEI software to move to 0, it rotates exactly back to 0. St... @Maria Janssen

12/01/2015

06:47 PM Leginon Leginon: Stage does not rotate back to zero after doing z-height measurements

Hi Anchi,
I was wondering if I have a microscope problem or a problem within Leginon... During z-height measuremen... @Maria Janssen

05/22/2015

04:58 PM Leginon Leginon: image orientaion

Hi Anchi,
Something weird happened in the past month where the reference image does not have the same orientation ... @Maria Janssen

08/05/2014

01:04 PM Leginon Leginon: RE: energy filter parameters

Ok. ....Sorry for the confusion: the microscope will be operated in EFTEM mode during the entire data collection, so ... @Maria Janssen

08/04/2014

04:55 PM Leginon Leginon: energy filter parameters

Hi,
I would like to use the energy filter only in exposure mode (ed), not during hole or square targeting. For thi... @Maria Janssen

02:25 PM Leginon Leginon: RE: Normalization correct after image rotation?

Hi Anchi,
Thanks for your help last Friday! I think I solved the problem we had:
1) The squares we see in the l... @Maria Janssen

07/24/2014

10:22 AM Leginon Leginon: Normalization correct after image rotation?

Hi!
Our DMS is set up as to have no flip and a 90 degrees rotation (post-gif k2 set-up). This gave some problems w... @Maria Janssen

07/22/2014

04:05 PM Leginon Leginon: focus problem

H!
I am sorry to come back to this again, but I keep having the same problem with focussing. As soon as I submit t... @Maria Janssen