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Bug #1327

open

discrepancy in image pixel size

Added by David Veesler over 13 years ago. Updated over 10 years ago.

Status:
New
Priority:
Normal
Assignee:
-
Category:
Image Processing
Start date:
06/13/2011
Due date:
% Done:

0%

Estimated time:
Affected Version:
Appion/Leginon 2.2.0
Show in known bugs:
No
Workaround:

Description

I got the following error when trying to make a stack with tilted images from a RCT experiment:
!!! WARNING: skipped 1 of 973 images
... [[ 0 no reprocess | 1 rejected | 0 wrong tilt | 0 in donedict ]]
... Process images old to new
... Stack pixelsize = 1.688 A
... Image pixelsize 6.753 A != Stack pixelsize 1.688 A
... Problem image name: 11jun09a_022gr_043sq_02cs_07en_01

Traceback (most recent call last):
File "/opt/appion/bin/makestack2.py", line 1145, in ?
makeStack.run()
File "/usr/lib64/python2.4/site-packages/appionlib/appionLoop2.py", line 47, in run
self.preLoopFunctions()
File "/opt/appion/bin/makestack2.py", line 985, in preLoopFunctions
self.checkPixelSize()
File "/opt/appion/bin/makestack2.py", line 55, in checkPixelSize
apDisplay.printError("This particle selection run contains images of varying pixelsizes, a stack cannot be created")
File "/usr/lib64/python2.4/site-packages/appionlib/apDisplay.py", line 56, in printError
raise Exception, colorString("\n * FATAL ERROR *\n"+text+"\n\a","red")
Exception: * FATAL ERROR *
This particle selection run contains images of varying pixelsizes, a stack cannot be created

In fact, when checking the indicated image I realized that while the tilted images has a pixel size of 1.69A/pixel as all the other in the dataset (collected at 50,000x) but the corresponding untilted one has 6.75A/pixel.
Thanks a lot
David

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