Feature #1867
closedMake a smarter Appion implementation of CtfFind
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Updated by Neil Voss over 12 years ago
- Status changed from Assigned to In Code Review
- Assignee changed from Neil Voss to Dmitry Lyumkis
Updated by Gabriel Lander over 12 years ago
Data that is uploaded (we are doing lots of film at the moment) does not have have a value for:
imgdata['camera']['pixel size']['x']
so that "dstep" value is set to None and ctffind won't run. I don't know if this should be fixed in the upload script, but for now I added some code to get the dstep from the uploaded pixelsize if the info from the camera comes back with None.
r16874
Updated by Gabriel Lander over 12 years ago
The method for determining the min & max defocus seems a bit excessive using the defaults of 5000 Angstroms and 25 steps in either direction.
For a nominal defocus of 2 microns, this will search from 0.5uM to 14.5uM
Is this intentional?
Updated by Neil Voss over 12 years ago
Hi Gabe. Not intentional, I was probably off by an order of magnitude. Make the change if you wish, otherwise I will try and get to it tomorrow.
Updated by Neil Voss over 12 years ago
Hi Gabe, I think your fix for the dstep is good. Rather than taking pixel size directly, ctffind requires two values and then calculates the pixel size, so as long as it works out it is okay with me.
Updated by Amber Herold over 12 years ago
- Target version changed from Appion/Leginon 2.3.0 to Appion/Leginon 2.2.1
Updated by Amber Herold over 12 years ago
- Target version changed from Appion/Leginon 2.2.1 to Appion/Leginon 3.0.0
Updated by Anchi Cheng almost 7 years ago
- Status changed from In Code Review to Closed