Appion

Tried to run ctfestimate.py, results sometimes gave positive and sometimes gave negative defocus values.

After looking at log file, found defaulted search range is set between -74896 and 25104 which doesn't seem to make sense.

Also, I don't know how to interpret the new display. (attached)

thanks!

Logfile:

CTF DETERMINATION, V3.5 (7-March-2012)

Distributed under the GNU General Public License (GPL) Input image file name
12jun18b_c_035gr_03sq_02hl_09ed.mrc Output diagnostic file name
12jun18b_c_035gr_03sq_02hl_09ed-pow.mrc CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]
2.0 200.0 0.07 42134.8 6.000 Positive defocus values for underfocus
Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A]
256 100.0 12.0 -74896.0 25104.0 2000.0 500.0 Opening MRC/CCP4 file for READ...
File : 12jun18b_c_035gr_03sq_02hl_09ed.mrc
WARNING: NO MACHINE STAMP
NX, NY, NZ: 4096 3072 1
MODE : real
Min, max : 5475.043 8719.488
Mean, RMS : 7644.072 800.0270 READING IMAGE...
NX, NY= 4096 3072 TILING IMAGE... Total tiles and number used 192 191
Lower resolution limit reset to 50.00000 A FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC -74000.00 -74000.00 0.00 0.01626
-72000.00 -74000.00 0.00 0.02031
-72000.00 -72000.00 0.00 0.03109
-72000.00 -70000.00 0.00 0.03512
-70000.00 -70000.00 0.00 0.04296
-60000.00 -58000.00 0.00 0.04732
-48000.00 -48000.00 0.00 0.05407
-48000.00 -46000.00 0.00 0.06787
-46000.00 -46000.00 0.00 0.06946
-40000.00 -40000.00 0.00 0.07285
-40000.00 -38000.00 0.00 0.08087
-38000.00 -38000.00 0.00 0.08941
-36000.00 -38000.00 0.00 0.09699
-36000.00 -36000.00 0.00 0.10241
-34000.00 -34000.00 0.00 0.10833
-34000.00 -32000.00 0.00 0.11743
-32000.00 -32000.00 0.00 0.12775
-30000.00 -32000.00 0.00 0.14162
-30000.00 -30000.00 0.00 0.15800
-28000.00 -30000.00 0.00 0.16059
-28000.00 -28000.00 0.00 0.17366
-26000.00 -28000.00 0.00 0.17690
-28000.00 -26000.00 0.00 0.17762
-26000.00 -26000.00 0.00 0.19700
-24000.00 -26000.00 0.00 0.19987
24000.00 24000.00 0.00 0.22364 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24191.66 24551.64 20.55 0.22593 Final Values Opening MRC/CCP4 file for WRITE...
File : 12jun18b_c_035gr_03sq_02hl_09ed-pow.mrc
NX, NY, NZ: 256 256 1
MODE : real