Appion

Found this from a highly stigamtic image, attached.

Running through Appion ctffind3 gives

logfiles final ctf DFMID1 9171.05, DFMID2 5113.67, ANGAST 55.54
ctfvalues.log nominal=1.0e-6, bestdef=5.1e-7, def_1=9.2e-07, def_2=5.1e-7, astig_angle=34.4, cross_corr=0.153
imageviewer shows def_1=5.1e-7, def_2=9.2e-7, astig_angle=-55.57

2D and 1D plot from Appion shows good match.

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Running through Appion ctffind4 gives
ctffind4 output:

• Estimated defocus values:9159.57, 5109.79 Angstroms
• Estimated azimuth of astigmatism: 34.18 degrees

database record and imageviewer shows
def_1=0.51 um, def_2=0.92 um, astig_amgle=34.18

2D and 1D plots appears mirrored between fit and data.

This bug is in 3.2, need fixing immediately.